Coding

Part:BBa_K598018

Designed by: Qingyang XIAO   Group: iGEM11_Peking_R   (2011-09-27)


tetA+GFP fused protein


the most important protein in the selection system, wthich is a confusing protein including both tetA and GFP coading sequences and a linking sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 18
    Illegal BamHI site found at 311
    Illegal BamHI site found at 1786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 705
    Illegal NgoMIV site found at 865
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologya fusion protein in which tetA, which encodes a tetracycline/H+ antiporter, was fused with a green fluorescent protein via a flexible peptide linker
functionThe most critical component of dule genetic selection, marker of ON/OFF selection and reporter of screen
n/atetA+GFP fused protein